Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of Bacillus amyloliquefaciens FZB42 wildtype and sigD- mutants grown in media supplemented with soil extract

ABSTRACT: Bacillus amyloliquefaciens FZB42 is a well-studied Gram-positive plant growth-promoting rhizobacteria. In this work we aim to study the effect of the effect of sigD deletion on the transcriptome of FZB42. The transcritomes were compared by two-color microarray of the sigD- mutant and the wildtype of B. amyloliquefaciens FZB42 grown in 1C medium supplemented with soil extract (SE). This submission includes data from two independent experiments with three biological replicates. Here a biological replicate means the bacterial culture from one flask used for RNA preparation. The samples were collected by two performers. The experiments were varied in sample performer, the date of the experiment.

ORGANISM(S): Bacillus amyloliquefaciens

SUBMITTER: Ben Fan

PROVIDER: E-MTAB-4876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

SigD vs WT in both SE and RS media

Project description:Bacillus amyloliquefaciens FZB42 is a well-studied Gram-positive plant growth-promoting rhizobacteria. In this work we aim to study the effect of the effect of sigD deletion on the transcriptome of FZB42. The transcritomes were compared by two-color microarray of the sigD- mutant and the wildtype of B. amyloliquefaciens FZB42 grown in 1C medium supplemented with soil extract (SE) and in 1C medium supplemented with both SE and maize root exudates (RE). This submission includes data from three independent experiments with three biological replicates. Here a biological replicate means the bacterial culture from one flask used for RNA preparation. The samples were collected by two performers. The experiments were varied in sample performer, the date of the experiment.

2017-07-05 | E-MTAB-4636 | biostudies-arrayexpress

Borriss_FZB42_soil extract

Project description:Transcription profiling by array of Bacillus amyloliquefaciens strain FZB42 after soil extract treatment, at OD600=1.0 and OD600=3.0 respectively.

2012-04-15 | E-MEXP-3551 | biostudies-arrayexpress

Borriss_FZB42_Root Exudates_OD1.0

Project description:Transcriptional profiling by array of Bacillus amyloliquefaciens strain FZB42 after root exudate treatment (0.25 g/L) at OD600=1.0<br><br>

2012-04-15 | E-MEXP-3550 | biostudies-arrayexpress

Borriss_FZB42_IE-RE

Project description:Transcription profiling by array of Bacillus amyloliquefaciens strain FZB42 after interaction exudates (IE) treatment, at OD600=1.0 and at OD600=1.0 respectively. IE was the root exudates prepared from maize plants growing with FZB42. The reference was treated with the root exudates (RE), prepared from maize plants grown in an axenic system.

2012-04-15 | E-MEXP-3553 | biostudies-arrayexpress

Differential RNA-seq of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42 Reveals sRNA Bas01 involved in Sporulation and Biofilm Formation

Project description:Bacillus amyloliquefaciens FZB42 is a representative organism for Gram positive soil bacteria associated with plant roots and beneficial to plant growth. It is of immense importance to understand mechanisms of this class of bacteria adapting to rhizosphere. In this work employing differential RNA sequencing (RNA-seq) and Northern blot, we systematically identified transcription start sites of mRNAs as well as non-coding regulatory RNAs in FZB42. The genes regulated at different growth phases and located in polycistronic operons were also identified. A set of genes were re-annotated. In addition, a sRNA named Bas01 was identified to be involved in Bacillus sporulation and biofilm formation. The result we obtained provides valuable data for investigation of Bacillus gene expression and molecular details of rhizobacterial interaction with host plants. Examination of transcriptome profile of rhizobacterium B. amyloliquefaciens FZB42 grown under six conditions.

2015-10-28 | E-GEOD-66681 | biostudies-arrayexpress

maize seed exudates - B. amyloliquefaciens FZB42

Project description:The effect of maize seed (Zea mays L. var. Surprise) exudates on Bacillus amyloliquefaciens FZB42 cultures grown to OD600 = 1.0 or 3.0 was tested against a negative seed exudate control sample.

2013-03-09 | E-MEXP-3788 | biostudies-arrayexpress

Borriss_FZB42_pilot experiment

Project description:Transcriptional profiling by array of Bacillus amyloliquefaciens FZB42 at log phase(OD600=1.0) and early stationary phase(OD600=3.0) after maize root exudate treatment of three concentrations(0.25mg/ml,0.50mg/ml,1.00mg/ml).

2012-04-15 | E-MEXP-3554 | biostudies-arrayexpress

Differential RNA-seq of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42 Reveals sRNA Bas01 involved in Sporulation and Biofilm Formation

2015-10-28 | GSE66681 | GEO

Bacillus amyloliquefaciens FZB42 maize root exudate treatment

Project description:The transcriptomic response of Bacillus amyloliquefaciens FZB42 to maize root exudates at OD600=3.0. This is a comprehensive analysis using the data of six microarray experiments (Exp1-2-3 and ExpABC respectively, 18 hybridization in total).

2011-11-01 | E-MEXP-3421 | biostudies-arrayexpress

RNA-Seq analysis of transcriptomes of B. amyloliquefaciens FZB42 in biofilm formation and planktonic cells.

Project description:This experiment compares the transcriptomes of B. amyloliquefaciens FZB42 cells in biofilm formation and in planktonic cells focusing on transcripts that were most affected in their transcription. The aim of the experiment was determination of the highest expressed genes in both cultures using RPKM values and comparison of relative transcription rates using DeSeq analysis.

2017-03-16 | E-MTAB-4362 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data