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Dysregulated immune system networks in war veterans with Post-Traumatic Stress Disorder (PTSD) is an outcome of altered miRNA expression

ABSTRACT: miRNA microarray with four controls and eight PTSD patients were included in the study. This study was performed to see if there is any alteration in the miRNA expression profile in the peripheral blood mononuclear cells (PBMCs) of PTSD patients (war veterans in this case). Microarray for the miRNAs was performed by Johns Hopkins Memorial Institute (Deep Sequencing and Microarray Core Facility), Baltimore. Total RNA, including mRNA, miRNA and other small RNA molecules, were isolated from PBMC samples by using total RNA isolation kit (AllPrep DNA/RNA/miRNA Universal Kit) from Qiagen. Next, total RNA samples were used in the analysis of miRNA differential expression by miRNA array hybridization assay using the Affymetrix miRNA-v1 gene chip. Linear fold-changes in miRNA up-regulation or down-regulation were calculated to compare the differences of all the miRNAs expressed between PTSD patients and controls.

ORGANISM(S): Homo sapiens

SUBMITTER: Mitzi Nagarkatti

PROVIDER: E-MTAB-4880 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dysregulated immune system networks in war veterans with PTSD

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