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In situ Hi-C study of chromatin conformation during early Drosophila development

ABSTRACT: In this experiment we examined higher order chromatin structure during early Drosophila melanogaster development. We performed in situ Hi-C for hand-sorted non-mitotic embryos at nuclear cycle number 12, 13 and 14, and for embryos at 3-4 hours post fertilisation. During this time in development, the zygotic genome is activated and zygotic transcription is taking place for the first time. To assess the impact of transcription on chromatin structure we injected the transcription inhibitors alpha-amanitin or triptolide before zygotic genome activation and performed Hi-C and ChIP-seq for RNA Pol II. Furthermore, we used Hi-C to study genome architecture in embryos lacking the transcription factor Zelda.

INSTRUMENT(S): Illumina MiSeq, NextSeq 500

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Clemens Hug

PROVIDER: E-MTAB-4918 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatin Architecture Emerges during Zygotic Genome Activation Independent of Transcription.

Hug Clemens B CB Grimaldi Alexis G AG Kruse Kai K Vaquerizas Juan M JM

Cell 20170401 2

Chromatin architecture is fundamental in regulating gene expression. To investigate when spatial genome organization is first established during development, we examined chromatin conformation during Drosophila embryogenesis and observed the emergence of chromatin architecture within a tight time window that coincides with the onset of transcription activation in the zygote. Prior to zygotic genome activation, the genome is mostly unstructured. Early expressed genes serve as nucleation sites for ...[more]

PMID: 28388407

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