Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Arabidopsis shoots and roots in response to exogenous H2O2 with differential kinetics

ABSTRACT: Arabidopsis plants (Col-0) were hydroponically grown for 10 days at 22°C under 30~40 μE·m−2·s−1 and continuous light conditions. Treatment with 100 mM H2O2 or sterile distilled water as mock control by spraying for shoot treatments, or dipping for root treatments. Relationship between treated and harvested tissue is as follows; shoot treatment-shoot sampling was analyzed at 1, 3, 6, 12, and 24h after the treatment, shoot treatment-root sampling, root treatment-shoot sampling, and root treatment-root sampling was analyzed at 6h after the treatment. Two biological replicates for each treatment were done by dye swaps (flip dyes). The data sets were normalized and filtered for quality control with GeneSpring12.5 (Agilent Technologies) using the default settings.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yoshiharu Yamamoto 

PROVIDER: E-MTAB-4961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is a common signal molecule initiating transcriptional responses to all the known biotic and abiotic stresses of land plants. However, the degree of involvement of H<sub>2</sub>O<sub>2</sub> in these stress responses has not yet been well studied. Here we identify time-dependent transcriptome profiles stimulated by H<sub>2</sub>O<sub>2</sub> application in Arabidopsis (<i>Arabidopsis thaliana</i>) seedlings. Promoter prediction based on transcriptom  ...[more]

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