Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq transcriptome profiling of the psychrophilic phytoplankton Fragilariopsis cylindrus under environmentally relevant growth limitations


ABSTRACT: We performed RNA-sequencing experiments to examine the differential regulation of genes in the genome of the Southern Ocean diatom Fragilariopsis cylindrus including diverged alleles. RNA-seq was performed on three replicate samples for each experimental condition. Phytoplankton cells were grown under six different experimental conditions including (1) optimal growth, (2) freezing temperatures, (3) elevated temperature, (4) elevated carbon dioxide concentrations, (5) low iron concentrations and (6) prolonged darkness. Total RNA was extracted using a guanidinium thiocyanate-phenol-chloroform extraction protocol, followed by DNase I treatment and RNA purification (Quiagen). First strand cDNA synthesis was performed using random hexamers. Library preparation was performed using the RNA-seq Sample Prep Kit (Illumina) and sequencing was conducted according to the TruSeq RNA sequencing protocol (Illumina) All samples were sequenced together in one flowcell on one lane.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Fragilariopsis cylindrus

SUBMITTER: Thomas Mock 

PROVIDER: E-MTAB-5024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Evolutionary genomics of the cold-adapted diatom Fragilariopsis cylindrus.

Mock Thomas T   Otillar Robert P RP   Strauss Jan J   McMullan Mark M   Paajanen Pirita P   Schmutz Jeremy J   Salamov Asaf A   Sanges Remo R   Toseland Andrew A   Ward Ben J BJ   Allen Andrew E AE   Dupont Christopher L CL   Frickenhaus Stephan S   Maumus Florian F   Veluchamy Alaguraj A   Wu Taoyang T   Barry Kerrie W KW   Falciatore Angela A   Ferrante Maria I MI   Fortunato Antonio E AE   Glöckner Gernot G   Gruber Ansgar A   Hipkin Rachel R   Janech Michael G MG   Kroth Peter G PG   Leese Florian F   Lindquist Erika A EA   Lyon Barbara R BR   Martin Joel J   Mayer Christoph C   Parker Micaela M   Quesneville Hadi H   Raymond James A JA   Uhlig Christiane C   Valas Ruben E RE   Valentin Klaus U KU   Worden Alexandra Z AZ   Armbrust E Virginia EV   Clark Matthew D MD   Bowler Chris C   Green Beverley R BR   Moulton Vincent V   van Oosterhout Cock C   Grigoriev Igor V IV  

Nature 20170116 7638


The Southern Ocean houses a diverse and productive community of organisms. Unicellular eukaryotic diatoms are the main primary producers in this environment, where photosynthesis is limited by low concentrations of dissolved iron and large seasonal fluctuations in light, temperature and the extent of sea ice. How diatoms have adapted to this extreme environment is largely unknown. Here we present insights into the genome evolution of a cold-adapted diatom from the Southern Ocean, Fragilariopsis  ...[more]

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