Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptional changes in dormant potato minitubers treated with 1-(alpha-ethylbenzy)-3-nitroquanidine

ABSTRACT: RNA was sequenced from commercially grown greenhouse minitubers treated with 10 ug of 1-(alpha-ethylbenzy)-3-nitroquanidine (NG) in DMSO and followed for four, and seven days post treatment. Control tubers were injected with DMSO alone. Sequence reads were mapped to the potato genome (PGSC_DM_v3_2.1.11) and transcript abundance was determined using Cuffdiff v 2.02. Overall design: Minitubers were injected with DMSO (control) or 10 ug of NG and then trancript changes were examined at on, four, and seven days after exposure.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Solanum tuberosum

SUBMITTER: Michael Campbell

PROVIDER: E-MTAB-5038 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Treatment of potato tubers with the synthetic cytokinin 1-(α-ethylbenzyl)-3-nitroguanidine results in rapid termination of endodormancy and induction of transcripts associated with cell proliferation and growth.

Campbell Michael M Suttle Jeffrey J Douches David S DS Buell C Robin CR

Functional & integrative genomics 20141001 4

Perennial plants undergo repression of meristematic activity in a process called dormancy. Dormancy is a complex metabolic process with implications for plant breeding and crop yield. Endodormancy, a specific subclass of dormancy, is characteristic of internal physiological mechanisms resulting in growth suppression. In this study, we examine transcriptional changes associated with the natural cessation of endodormancy in potato tuber meristems and in endodormant tubers treated with the cytokini ...[more]

PMID: 25270889

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Project description:Gastrodia elata Blume (Orchidaceae) is an important Chinese medicine with several functional components. In the life cycle of G. elata, the orchid develops a symbiotic relationship with two compatible mycorrhizal fungi Mycena spp. and Armillaria mellea during seed germination to form vegetative propagation corm and vegetative growth to develop tubers, respectively. Gastrodin (p-hydroxymethylphenol-beta-D-glucoside) is the most important functional component in G. elata, and gastrodin significantly increases from vegetative propagation corms to tubers. To address the gene regulation mechanism in gastrodin biosynthesis in G. elata, a comparative analysis of de novo transcriptome sequencing among the vegetative propagation corms and tubers of G. elata and A. mellea was conducted using deep sequencing. Transcriptome comparison between the vegetative propagation corms and juvenile tubers of G. elata revealed 582 differentially expressed unigenes, of which 415 and 167 genes were, respectively up-regulated (fold-change â?¥ 2, p-value < 0.05) and down-regulated (fold-change â?¤ 0.5, p-value <0.05) in juvenile tubers. After Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, forty-seven up-regulated unigenes with enzyme commission (EC) were assigned to 269 isogroups involved in 100 different pathways, and twenty-four down-regulated unigenes with enzyme commission (EC) were assigned to 586 isogroups, involved in 167 different pathways. The analysis of the isogroup genes from all pathways revealed that the two unigenes at locus 25,051 (one of monooxygenases) and locus 22,288 (one of glycosyltransferases) might participate in hydroxylation and glucosylation in the gastrodin biosynthetic pathway. The gene expression of the two unique unigenes encoding monooxygenase and glycosyltransferase significantly increases from vegetative propagation corms to tubers, and the molecular basis of gastrodin biosynthesis in the tubers of G. elata is proposed. Transcriptome comparative between vegetative propagation corms and tubers of G. elata, and between tubers of G. elata (symbiosis A. mellea) and A. mellea

Dataset Information

Transcriptional changes in dormant potato minitubers treated with 1-(alpha-ethylbenzy)-3-nitroquanidine

Publications

Treatment of potato tubers with the synthetic cytokinin 1-(α-ethylbenzyl)-3-nitroguanidine results in rapid termination of endodormancy and induction of transcripts associated with cell proliferation and growth.

Similar Datasets

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