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Meiotic recombination initiation hotspots in Arabidopsis genes and transposons (MNase-seq)

ABSTRACT: To analyse nucleosome positioning and occupancy we performed micrococcal nuclease digestion of Arabidopsis wild type (Col-0) chromatin and gel purified the resulting ~150 bp mononucleosomal DNA band. This DNA was used to generate a library and paired-end sequencing performed (MNase-seq). To further examine influence of SWR1 chromatin remodelling complex and DNA methylation on nucleosome occupancy, we repeated MNase-seq in Aarabidopsis arp6 and met1 mutant.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Xiaohui Zhao

PROVIDER: E-MTAB-5042 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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