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A new strategy to identify the long-distance mobile peptides from xylem sap.

ABSTRACT: Total RNAs were extracted from internodes and roots of 18 day-after-germination soybean plants, and the expression levels of the genes that encode xylem sap associated peptides or proteins were analyzed.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Glycine max

SUBMITTER: Satoru Okamoto

PROVIDER: E-MTAB-5052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A comprehensive strategy for identifying long-distance mobile peptides in xylem sap.

Okamoto Satoru S Suzuki Takamasa T Kawaguchi Masayoshi M Higashiyama Tetsuya T Matsubayashi Yoshikatsu Y

The Plant journal : for cell and molecular biology 20151007 3

There is a growing awareness that secreted pemediate organ-to-organ communication in higher plants. Xylem sap peptidomics is an effective but challenging approach for identifying long-distance mobile peptides. In this study we developed a simple, gel-free purification system that combines o-chlorophenol extraction with HPLC separation. Using this system, we successfully identified seven oligopeptides from soybean xylem sap exudate that had one or more post-transcriptional modifications: glycosyl ...[more]

PMID: 26333921

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