Transcriptional response of LPS-stimulated monocyte-derived macrophages in the presence or absence of the anti-TNF agonist Etanercept
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ABSTRACT: We measured TNFα activity on the transcriptional level by identifying TNFα-inducible genes in human blood monocyte-derived macrophages (MDM). On the basis that TNFα does not act in isolation, we used bacterial lipopolysaccharide (LPS) as a prototypic model of a stimulus to invoke secretion of a wide range of immunologically active factors, including TNFα. In order to identify gene expression attributable to LPS-induced TNFα, we compared the transcriptome of MDM 24 hours after stimulation with 100 ng/ml ultra-pure LPS in the presence and absence of the soluble TNF receptor fusion protein Etanercept (10 µg/ml).
ORGANISM(S): Homo sapiens
SUBMITTER: Carolin Turner
PROVIDER: E-MTAB-5095 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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