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Gene expression between dry and imbibed seeds in vitamin C defective (vtc) mutants and wild-type Arabidopsis thaliana

ABSTRACT: In the dry state, orthodox seeds have little antioxidant capacity, relying on reduced glutathione and tocochromanols for antioxidant defences rather than ascorbate or ascorbate peroxidase (APX). Ascorbate is synthesised de novo within a few hours of imbibition resulting in rapid increases in the ascorbate pool in the developing embryo. The knock-on effects of enhanced oxidation on embryo development before and after imbibition remain poorly characterised. A T-DNA insertional mutant line (SAIL_769_H05) line was used in these studies together with vtc2-1 mutants that were originally identified in an ethyl-methanesulfonate (EMS) mutagenesis screen. Expression levels of dry seeds and imbibed seeds of the genotypes Col0, vtc2 (EMS) and vtc2.5 (SAIL_769_H05) were quantified using Illumina RNA-seq technology

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Michael Wilson 

PROVIDER: E-MTAB-5103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Redox Changes During the Cell Cycle in the Embryonic Root Meristem of Arabidopsis thaliana.

de Simone Ambra A   Hubbard Rachel R   de la Torre Natanael Viñegra NV   Velappan Yazhini Y   Wilson Michael M   Considine Michael J MJ   Soppe Wim J J WJJ   Foyer Christine H CH  

Antioxidants & redox signaling 20170530 18

<h4>Aims</h4>The aim of this study was to characterize redox changes in the nuclei and cytosol occurring during the mitotic cell cycle in the embryonic roots of germinating Arabidopsis seedlings, and to determine how redox cycling was modified in mutants with a decreased capacity for ascorbate synthesis.<h4>Results</h4>Using an in vivo reduction-oxidation (redox) reporter (roGFP2), we show that transient oxidation of the cytosol and the nuclei occurred at G1 in the synchronized dividing cells of  ...[more]

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