Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq of the ileo-caecal valve lymph node of sheep with paratuberculosis compared to uninfected controls

ABSTRACT: Sheep total RNA was extracted from the Ileo-caecal valve lymph node (ICLN) of sheep with multibacillary paratuberculosis compared to ICLN of uninfected controls. Sequencing libaries were prepared from RNA using the Illumina TruSeq RNA Sample Preparation Kit v2. Sequencing with 101 base paired end reads was performed on an Illumina HiSeq 2500 at Edinburgh Genomics.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Ovis aries

SUBMITTER: John Hopkins

PROVIDER: E-MTAB-5146 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Pathways and Genes Associated with Immune Dysfunction in Sheep Paratuberculosis.

Gossner Anton A Watkins Craig C Chianini Francesca F Hopkins John J

Scientific reports 20170424

Multibacillary and paucibacillary paratuberculosis are both caused by Mycobacterium avium subspecies paratuberculosis. Multibacillary lesions are composed largely of infected epithelioid macrophages and paucibacillary lesions contain T cells but few bacteria. Multibacillary disease is similar to human lepromatous leprosy, with variable/high levels of antibody and a dysfunctional immune response. Animals with paucibacillary disease have high cell-mediated immunity and variable levels of antibody. ...[more]

PMID: 28436433

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Project description:The Bashbay sheep (Ovis aries), an indigenous breed of Xinjiang, China, has many excellent characteristics. However, hybrids of Argali sheep and Bashbay sheep are susceptible to Mycoplasma ovipneumoniae infection, the causative agent of mycoplasma ovipneumonia, a chronic respiratory disease that is harmful to the sheep industry. To date, knowledge regarding the mechanisms responsible for M. ovipneumoniae pathogenesis is in scant. Herein, we report the results of transcriptome profiling of lung tissues from Argali hybrid sheep experimentally infected with an M. ovipneumoniae strain at 4 and 14 days post-infection, in comparison to mock-infected animals (0 d). Transcriptome profiling was performed by deep RNA sequencing, using the Illumina platform. The analysis of differentially expressed genes was performed to determine concomitant gene-specific temporal patterns of mRNA expression in the lungs after M. ovipneumoniae infection. We found 156 differentially expressed genes (44 up-regulated, 112 down-regulated) when comparing transcriptomic data at 4 and 0 days post-infection, and 367 (35 up-regulated, 332 down-regulated) when comparing 14 versus 0 days post-infection. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the differentially expressed genes at 4 and 14 versus 0 days post-infection were enriched in 109 and 150 pathways, respectively, and the Primary immunodeficiency pathway was considered most closely related to MO infection (p < 0.01). Hyper-IgM syndrome was identified based on the B-cell immunodeficiency signaling pathway from differentially expressed genes related M. ovipneumoniae infection. Gene Ontology analysis showed that differentially expressed genes in different groups were enriched for 497 and 928 terms, where those most closely related to M. ovipneumoniae infection are ciliated motor damage (p < 0.01). These results could aid in understanding why the Argali hybrid sheep are susceptible to MO as well as how M. ovipneumoniae infection progresses in the lungs and may provide useful information regarding key regulatory pathways.

Dataset Information

RNA-seq of the ileo-caecal valve lymph node of sheep with paratuberculosis compared to uninfected controls

Publications

Pathways and Genes Associated with Immune Dysfunction in Sheep Paratuberculosis.

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