Transcriptomic data from early stages during zygotic embryo development in Pinus sylvestris L.

ABSTRACT: Embryos and megagametophytes samples belonging to four early developmental stages during seed development in Pinus sylvestris were collected from a seed orchard in central Sweden. Dominant (DO) and subordinate (SU) zygotic embryos were collected separately. RNA was isolated from this samples and the corresponding cDNA synthesized and subjected to RNA sequencing (one biological replicate). In total nine RNA-seq libraries were constructed, five for embryo samples (E1, E2, E3DO, E3SU, E4) and four for megagametophytes (M1, M2, M3 and M4). qRT-PCR analysis have been done to validate the RNA-seq expression results.

INSTRUMENT(S): 454 GS FLX+

ORGANISM(S): Pinus sylvestris

SUBMITTER: Lieven Sterck 

PROVIDER: E-MTAB-5193 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress