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Transcriptomic analyses of anti-adipogenic effects of oleuropein in human mesenchymal stem-cells

ABSTRACT: The main goal of the present work was to use an in vitro adipogenic-differentiation mode, with mesenchymal stem-cells (MSC) from human bone-marrow, to study whole-transcriptomic differential gene-expression when such cells are induced to differentiate into adipocytes in absence or presence of oleuropein. See details below. This work is complementary to a previously published one (Casado-Daz et al, 2016): Casado-DÃaz, A., Anter, J., MÃ¼ller, S., Winter, P., Quesada-GÃ³mez, J. M. and Dorado, G. (2016): Transcriptomic analyses of adipocyte differentiation from human mesenchymal stromal-cells (MSC). Journal of Cellular Physiology 9999: 1-14. DOI: 10.1002/jcp.25472. It corresponds to EMBL-EBI ArrayExpress accession E-MTAB-4884.

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Homo sapiens

SUBMITTER: Nicolas Krezdorn

PROVIDER: E-MTAB-5211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic analyses of adipocyte differentiation from human mesenchymal stromal-cells (MSC)

Project description:Adipogenesis is a physiological process required for fat-tissue development, mainly involved in regulating the organism energetic-state. Abnormal distribution-changes and dysfunctions in such tissue are associated to different pathologies. Adipocytes are generated from progenitor cells, via a complex differentiating process not yet well understood. Therefore, we investigated differential mRNA and miRNA expression patterns of human mesenchymal stromal-cells (MSC) induced and not induced to differentiate into adipocytes by next (second)-generation sequencing. A total of 2,866 differentially-expressed genes (101 encoding miRNA) were identified, with 705 (46 encoding miRNA) being upregulated in adipogenesis. They were related to different pathways, including PPARG, lipid, carbohydrate and energy metabolism, redox, membrane-organelle biosynthesis and endocrine system. Downregulated genes were related to extracellular matrix and cell migration, proliferation and differentiation. Analyses of mRNA-miRNA interaction showed that repressed miRNA-encoding genes can act downregulating PPARG-related genes; mostly the PPARG activator (PPARGC1A). Induced miRNA-encoding genes regulate downregulated genes related to TGFB1. These results shed new light to understand adipose-tissue differentiation and physiology, increasing our knowledge about pathologies like obesity, type-2 diabetes and osteoporosis.

2016-08-31 | E-MTAB-4884 | biostudies-arrayexpress

Regulation of the cardiac transcriptome by the E3 ubiquitin ligase Huwe1.

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2023-11-23 | E-MTAB-13494 | biostudies-arrayexpress

Variations of secretome profiles according to conditioned medium preparation: the example of human mesenchymal stem cell derived adipocytes

Project description:One challenging point in analysing cellular secretome collected as conditioned medium is cross contamination by cell culture media components, especially bovine serum proteins. A common approach for serum removal is to wash the cells, an alternative is to grow cells using serum-free conditions. Given that the sample processing may influence the phenotype of cells and thus the secretome, it is important to establish the optimal protocol for each cell type. In this study, we compared two methods for preparing conditioned medium from human adipocytes derived from mesenchymal stem cells. Cells were either washed twice with PBS or cultured the last four days of differentiation in serum free adipogenic medium. Gene expression of the cells was evaluated by using real time PCR and one dimensional LC-MS/MS was used to compare secreted proteins present in the culture supernatants. Surprisingly, results showed significant differences in gene expression patterns of the cells and in protein content of the conditioned media and suggested that PBS washes induced severe modifications of the phenotype of cells and thus changes in protein secretion profiles. These data emphasize the significant variations in protein species related to cell manipulations and underline the importance of procedure optimization prior to any proteomic investigation.

2015-07-03 | PXD002217 | Pride

Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues

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2019-07-20 | E-MTAB-6646 | biostudies-arrayexpress

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2024-01-31 | E-MTAB-11546 | biostudies-arrayexpress

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2023-12-11 | E-MTAB-12988 | biostudies-arrayexpress

Human iPSC-derived cardiomyocytes of peripartum cardiomyopathy patients reveal aberrant expression of sterol and lipid metabolism pathways

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