Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

31 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq study of 3-days larva and pupa in Lysiphlebia japonica

ABSTRACT: Parasitoids were considered to have the ability to synthesize the lipid. The cotton aphids were parasitized by Lysiphlebia japonica, and Lysiphlebia japonica obtained lipids from cotton aphids. In our study, we get the 3 days larva and pupa from cotton aphids and analysis the expression of the genes involved in the lipid related pathway of these two developmental stages.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Lysiphlebia japonica

SUBMITTER: Gao Xueke

PROVIDER: E-MTAB-5227 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

RNA-Seq study of lipid regulation in Aphis gossypii parasitized by Lysiphlebia japonica

Project description:Parasitoids were considered to have the ability to synthesize the lipid. The cotton aphids were parasitized by Lysiphlebia japonica, and Lysiphlebia japonica obtained lipids from cotton aphids. In our study,we get the 3 days parasitized aphids and unparasitized aphids and analysis the expression of the genes involved in the lipid related pathway.

2018-10-10 | E-MTAB-5228 | biostudies-arrayexpress

Lipidomics and RNA-Seq Study of Lipid Regulation in Aphis gossypii parasitized by Lysiphlebia japonica

Project description:The cotton-melon aphid, Aphis gossypii Glover, is a major insect pest worldwide. Lysiphlebia japonica (Ashmead) is an obligate parasitic wasp of A. gossypii, and has the ability to regulate lipid metabolism of the cotton-melon aphid. Lipids are known to play critical roles in energy homeostasis, membrane structure, and signaling. However, the parasitoid genes that regulate fat metabolism and lipid composition in aphids are not known. 34 glycerolipids and 248 glycerophospholipids were identified in this study. We have shown that a 3-day parasitism of aphids can induce significant changes in the content and acyl chain composition of triacylglycerols (TAGs) and subspecies composition of glycerophospholipids content and acyl chains. It also upregulate the expression of several genes involved in triacylglycerol synthesis and glycerophospholipid metabolism. Pathway analysis showed that a higher expression of genes involved in the tricarboxylic acid cycle and glycolysis pathways may contribute to TAGs synthesis in parasitized aphids. Interestingly, the higher expression of genes in the sphingomyelin pathway and reduced sphingomyelin content may be related to the reproductive ability of A. gossypii. We provide a comprehensive resource describing the molecular signature of parasitized A. gossypii particularly the changes associated with the lipid metabolism and discuss the biological and ecological significance of this change.

2018-03-07 | MTBLS410 | MetaboLights

Microarray-based Gene Expression Analysis in the Silkworm Bombyx mori After Parasitization by Tachinid Flies

Project description:We identified genes regulated by parasitization of the silkworm Bombyx mori by three tachinid parasitoid species, Exorista japonica, Drino inconspicuoides and Pales pavida, using oligonucleotide microarrays. The numbers of genes and their intensity of expression varied with the species of parasitoid, within silkworm hemocytes and fat body. Bombyx mori hemocyte, silkgland and fat body samples parasitizated by Exorista japonica, Drino inconspicuoides and Pales pavida were prepared. Gene expression was compared in these two groups: control and parasitized.

2015-09-17 | E-GEOD-73085 | biostudies-arrayexpress

Project description:Microbiome diversity of cotton aphids

| PRJNA591256 | ENA

RNA-Seq study of lipid regulation in Aphis gossypii parasitized by Lysiphlebia japonica

Project description:RNA-Seq study of lipid regulation in Aphis gossypii parasitized by Lysiphlebia japonica

| PRJEB17746 | ENA

Project description:RNA-Seq of cotton leaves induced by cotton aphids

| PRJNA576973 | ENA

Project description:Transcriptome sequencing (RNAseq) of pea aphids parasitized with wasp larvae

| PRJNA667506 | ENA

Expression of small RNA in Aphis gossypii and its potential role in the resistance interaction with melon

Project description:This study was designed to identify the sRNAs in Aphis gossypii (cotton-melon aphid) during Vat-mediated resistance in teraction with melon Methods: Whole insects were collected from susceptible (Vat-) and resistant (Vat+) plants after 48 hours of feeding. Total RNA was extracted from the aphids and enriched for LMW RNA and small RNA libraries were constructed using standard protocols and deep sequenced using Illumina GAII analyzer.

2013-06-01 | E-GEOD-38641 | biostudies-arrayexpress

Dual scRNA-Seq analysis reveals rare and uncommon parasitized cell populations in chronic L. donovani infection

Project description:Although phagocytic cells are documented targets of Leishmania parasites, it is unclear whether other cell types can be infected. Here, we use unbiased scRNA-seq to simultaneously analyze host cell and Leishmania donovani transcriptomes to identify and annotate parasitized cells in spleen and bone marrow in chronically infected mice. Our dual-scRNA-seq methodology allows the detection of heterogenous parasitized populations. In the spleen, monocytes and macrophages are the dominant parasitized cells, while megakaryocytes, basophils and NK cells are found unexpectedly infected. In the bone marrow, the Hematopoietic Stem Cells (HSCs) expressing phagocytic receptors FcγR and CD93 are the main parasitized cells. Additionally, we also detect parasitized cycling basal cells, eosinophils, and macrophages in chronically infected mice. Flow cytometric analysis confirms the presence of parasitized HSCs. Ourunbiased dual scRNA-seq method identifies rare, parasitized cells, possibly implicated in pathogenesis, persistence, and protective immunity using a non-targeted approach.

2023-09-14 | GSE240385 | GEO

Aphis gossypii

Project description:Small RNA-seq analysis of miRNAs in cotton-melon aphids

| PRJNA495733 | ENA