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Activin/Nodal-dependent N6-methyladenosine (m6A) deposition in human pluripotent stem cells

ABSTRACT: This experiment was designed to probe the function of Activin/Nodal signalling in the deposition of m6A in human pluripotent stem cells (hPSCs). hPSCs were cultured in presence of Activin or subjected to short-term inhibition of Activin/Nodal signalling for 2h using the receptor antagonist SB-431542 (IP). The global abundance of m6A was then measured by nuclear-enriched methylated RNA immunoprecipitation followed by deep sequencing (NeMeRIP-seq). Pre-NeMeRIP input RNA was used as control to normalise for the changes in gene expression in the two conditions.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Pedro Madrigal Bayonas

PROVIDER: E-MTAB-5229 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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