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Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors.

ABSTRACT: They aim of the study is to identify targets of Carboxypeptidase E (CPE) as well as signaling cascades that are affected by CPE which are specific for transmitting its anti-migratory effects in glioma cells by overexpressing CPE and using transcriptomics profiling of mRNAs and microRNAs.

ORGANISM(S): Homo sapiens

SUBMITTER: Arnaud Muller

PROVIDER: E-MTAB-5299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors.

Project description:They aim of the study is to identify targets of Carboxypeptidase E (CPE) as well as signaling cascades that are affected by CPE which are specific for transmitting its anti-migratory effects in glioma cells by overexpressing CPE and using transcriptomics profiling of mRNAs and microRNAs.

2017-11-29 | E-MTAB-5297 | biostudies-arrayexpress

Gene expression of the human choroid plexus epithelium (CPE)

Project description:We investigated the gene expression of the human CPE.We isolated CPE cells from healthy human donor choroid plexus tissues with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, namely human RPE/choroid RNA. We performed 7 replicates of human CPE samples from 7 different donors.

2013-09-27 | E-GEOD-49969 | biostudies-arrayexpress

Gene expression of the mouse choroid plexus epithelium (CPE)

Project description:We investigated the gene expression of the mouse CPE. We isolated CPE cells from three mouse brains with laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44k Agilent microarrays. We performed the microarrays against a common reference sample, which was mouse RPE/choroid RNA. We performed 3 mouse CPE replicates from 3 different mouse from the same strain, namely C57BL/6 .

2013-09-27 | E-GEOD-49973 | biostudies-arrayexpress

Comparisons of the global gene profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells.

Project description:A microarray analysis was performed to compare the global gene expression profile between C-CPE treated- and untreated- SKOV-3 ovarian cancer cells. SKOV-3 cells were treated with or without C-CPE for 72 hours, and total RNA was extracted and microarray was perfomed to compare the gene profiling changes between C-CPE treated- and untreated- cells. The experiment was performed in triplicate.

2010-06-23 | E-GEOD-22421 | biostudies-arrayexpress

RNA-Seq of native and FusionRed-transfected canine tumor cell lines after 3 hours C-CPE treatment

Project description:Claudin (CLDN) proteins are commonly expressed in cancers and accordingly targeted in novel therapeutic approaches. C-terminus of Clostridium perfringens enterotoxin (C-CPE) binds efficiently several claudins and can be used to specifically target cancer cells. This experiment used RNA-seq to profile gene expression changes of native and FusionRed transfected canine tumor cell lines;TihoDTCC0840 (0840) and TihoDProAdCarc0846 (0846) after a 3 hours of C-CPE treatment (20µg/ml).

2021-11-30 | E-MTAB-10839 | biostudies-arrayexpress

A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation [RBP screening 293T cells]

Project description:We process mass spectrome datasets of RNA binding proteins(RBPs) in soluble nuclear extract (SNE) and chromatin-pellet extract (CPE) from 293T cells.

2021-09-27 | PXD028660 | Pride

A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation [RBP screening K562 cells]

Project description:We process mass spectrome datasets of RNA binding proteins(RBPs) in soluble nuclear extract (SNE) and chromatin-pellet extract (CPE) from K562 cells.

2021-09-27 | PXD028668 | Pride

A global screening identifies chromatin-enriched RNA-binding proteins and the transcriptional regulatory activity of QKI5 during monocytic differentiation [RBP screening THP-1 cells]

Project description:We process mass spectrome datasets of RNA binding proteins(RBPs) in soluble nuclear extract (SNE) and chromatin-pellet extract (CPE) from THP-1 cells.

2021-09-27 | PXD028787 | Pride

Expression data from human glioma cancer stem cell (CSC) specimens following knockdown of GREM1

Project description:CSCs differentially secrete the BMP antagonist Gremlin1 compared to non-stem glioma populations. Knockdown of Gremlin1 decreases CSC proliferation and tumorigenicity, establishing Gremlin1 as an essential effector for CSC maintenance. We used a microarray to determine the gene expression programs that are activated downstream of Gremlin1 that might be responsible for CSC maintenance. Glioma CSC cultures from two distinct patient-derived specimens (528 and 3691) were transduced with lentivirus expressing non-targeting scrambled shRNA or one of two distinct Gremlin1 shRNAs (shGrem1_485 and shGrem1_2456) for 72 hours (total of six samples). RNA was extracted for array hybridization.

2014-06-03 | E-GEOD-52846 | biostudies-arrayexpress

Gene expression comparison of MCF10A cells with and without extra centrosomes through overexpression of PLK4

Project description:The presence of extra centrosomes is a feature of human tumours. However, it is unclear what are the changes elicited by the presence of these abnormalities. To determine the changes in gene expression induced by centrosome amplification, human mammary epithelial cells, MCF10A, expressing a doxycycline inducible PLK4 cDNA were used. Centrosome amplification was induced for 48 hrs upon the addition of doxycyclin to the culture medium. RNA was purified from MCF10A cells without extra centrosomes (no dox) and with extra centrosomes (dox) and processed for microarray analysis.

2018-02-10 | E-MTAB-6415 | biostudies-arrayexpress

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