Single cell RNA sequencing from extra-thymic Aire-expressing cells (Cd31-gp38-Cd45+EpCam+MHC+GFP+) sorted from mixed/pooled lymph nodes from 2 C57 BL/6 mice (Homunculus project).
Ontology highlight
ABSTRACT: Aire-expressing cells have been detected across several non-thymic immune sites, including peripheral lymph nodes. The transcriptome of these extra-thymic Aire expressing cells (eTACs) will be used to indicate their ontogeny and predicted function and antigen presenting cells. In particular, it is not known whether these cells represent a single homogenous population, or a comprised of multiple different cell types. Single cell RNA sequencing allows us to characterise any heterogeneity in these cells.
INSTRUMENT(S): AutoMACS, Illumina HiSeq 2000
ORGANISM(S): Mus musculus
SUBMITTER: Mike Morgan
PROVIDER: E-MTAB-5362 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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