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RNA-seq data from the trachea epithelial cells of two inbred chicken lines at three time points after challenge with lentogenic Newcastle disease virus

ABSTRACT: This study aimed to characterize differential responses between the relatively resistant (Fayoumi) and susceptible (Leghorn) chicken lines when challenged with a high titered lentogenic Newcastle Disease Virus (NDV) strain via the eyes and nostrils at three weeks of age. The trachea epithelial cells were collected from challenged and non-challenged birds from each line at 2, 6, and 10 days-post-infection (dpi). High quality RNA was isolated, used for cDNA library construction, and sequenced on the HiSeq2500.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Gallus gallus

SUBMITTER: Melissa Deist

PROVIDER: E-MTAB-5431 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Novel Mechanisms Revealed in the Trachea Transcriptome of Resistant and Susceptible Chicken Lines following Infection with Newcastle Disease Virus.

Deist Melissa S MS Gallardo Rodrigo A RA Bunn David A DA Kelly Terra R TR Dekkers Jack C M JCM Zhou Huaijun H Lamont Susan J SJ

Clinical and vaccine immunology : CVI 20170505 5

Newcastle disease virus (NDV) has a devastating impact on poultry production in developing countries. This study examined the transcriptome of tracheal epithelial cells from two inbred chicken lines that differ in NDV susceptibility after challenge with a high-titer inoculum of lentogenic NDV. The Fayoumi line had a significantly lower NDV load postchallenge than the Leghorn line, demonstrating the Fayoumi line's classification as a relatively NDV-resistant breed. Examination of the trachea tran ...[more]

PMID: 28331077

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