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Time course RNA-seq of Mycobacterium tuberculosis exposed to nitric oxide.

ABSTRACT: Mycobacterium tuberculosis is an intracellular human pathogen with the ability to resist and adapt to many adverse conditions it encounters upon infection. Among these, overcoming the production of nitric oxide by macrophages could be key for M. tuberculosis success. We have challenged M. tuberculosis with a sub-lethal concentration of nitric oxide and followed the transcriptomic response through RNA-seq for 48 hours.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mycobacterium tuberculosis H37Rv

SUBMITTER: Teresa Cortes

PROVIDER: E-MTAB-5557 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Delayed effects of transcriptional responses in Mycobacterium tuberculosis exposed to nitric oxide suggest other mechanisms involved in survival.

Cortes Teresa T Schubert Olga T OT Banaei-Esfahani Amir A Collins Ben C BC Aebersold Ruedi R Young Douglas B DB

Scientific reports 20170815 1

Mycobacterium tuberculosis has succeeded as a human pathogen for tens of thousands of years thanks to its ability to resist and adapt to the adverse conditions it encounters upon infection. Bacterial adaptation to stress is commonly viewed in the context of transcriptional regulation, with the implicit expectation that an initial transcriptomic response is tightly coupled to an ensuing proteomic response. However, after challenging M. tuberculosis with nitric oxide we found that the rapid transc ...[more]

PMID: 28811595

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