Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Microarray analyses of microRNA expressions in comparison with immortal healthy mammary epithelial cells, breast cancer cells, and long-term-cultured breast cancer stem cells

ABSTRACT: RNA extraction and microarray analysis total RNA from immortalized normal mammary epithelial cells (184A1, MCF-12A), breast cancer cells (MDA-MB-231, MCF-7, MDA-MB-468, SK-BR-3), BCSC (MDA-MB-231SC, MCF-7SC, XM322, XM607). MDA-MB-231SC and MCF-7SC originating from breast cancer cell lines; XM322 and XM607 derived from clinical specimens which had been described in previous submission (E-MTAB-5057). The miRNA profiling was performed using Agilent miRNA array. Microarray experiments were conducted according to the manufacturer's instructions. To select the differentially expressed genes, we used threshold values of ≥ 2 and ≤ −2-fold change and a Benjamini-Hochberg corrected p value of 0.05. The data was Log2 transformed and median centered by genes using the Adjust Data function of Cluster 3.0 software then further analyzed with hierarchical clustering with average linkage (genes which value more than 100 were evaluated).

INSTRUMENT(S): Agilent 2100 bioanalyzer, NanoDrop ND-1000

ORGANISM(S): Homo sapiens

SUBMITTER: Xiaoti Lin

PROVIDER: E-MTAB-5584 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

POMC maintains tumor-initiating properties of tumor tissue-derived long-term-cultured breast cancer stem cells.

Lin Xiaoti X Chen Weiyu W Wei Fengqin F Zhou Binhua P BP Hung Mien-Chie MC Xie Xiaoming X Xie Xiaoming X

International journal of cancer 20170316 11

The identification and understanding of the molecular network of cancer stem cells (CSCs) have had a profound impact on our view of carcinogenesis and treatment strategy. Unfortunately, a major problem is that serial passages of CSCs from clinical solid tumor specimens currently are not available in any lab, and thus, reported data are difficult to confirm and intensively interrogated. Here, we have generated two tumor tissue-derived breast CSC (BCSC) lines that showed prolonged maintenance over ...[more]

PMID: 28214331

Publication: 1/2

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Dataset Information

Microarray analyses of microRNA expressions in comparison with immortal healthy mammary epithelial cells, breast cancer cells, and long-term-cultured breast cancer stem cells

Publications

POMC maintains tumor-initiating properties of tumor tissue-derived long-term-cultured breast cancer stem cells.

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