Gene expression profiles of control versus Prdm16-overexpressing cells

ABSTRACT: Embryonic neocortical cells were isolated from E14.5 WT mice, followed by TrypLE Express treatment and trituration to generate a single-cell suspension. Plasmid DNA was introduced into primary neocortical cells using Neon Transfection System. Next, neocortical neurospheres were cultured in serum-free media containing, then control and Prdm16-overexpressing cells were corrected after GFP sorting of 1 day cultures. We found that marked upregulation of modulators of mitochondrial metabolism and ROS balance in Prdm16 GOF cells

ORGANISM(S): Mus musculus

SUBMITTER: Ken-ichi Mizutani 

PROVIDER: E-MTAB-5646 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress