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Microarray transcription analysis of MDS/MPN leukocyte samples with and without EZH2 mutations (n = 12, each) and EZH2-downregulated F-36P, MOLM-13 and OCI-M2 cell lines, using 2 shRNAs targeting EZH2 and a non-targeting control shRNA, each

ABSTRACT: The aim of this study was to identify EZH2 targets in myeloid malignancies. RNA samples from control F-36P, MOLM-13 and OCI-M2 cells (secondary AML after MDS; EZH2 wild type) treated with random shRNA (n = 4, each) and test F-36P, MOLM-13 and OCI-M2 cells treated with either one of two EZH2-targeting shRNAs (n = 4, each) were screened for differential gene expression. RNA from EZH2 wild type (n = 12) and mutant (n = 12) MDS/MPN patients was also analyzed by microarray transcriptome analysis.

INSTRUMENT(S): Illumina iScan System, Human-HT-12-V4 Expression BeadChip, Biorad MJ Thermal Cycler (Biorad)

ORGANISM(S): Homo sapiens

SUBMITTER: Jenny Rinke

PROVIDER: E-MTAB-5766 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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