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Microarray transcription of murine lung and uterine group 2 innate lymphoid cells (ILC2s) cultured in vitro with or without 17beta-estradiol (estrogen, E2)

ABSTRACT: This experiment sought to determine whether estrogen could directly activate changes in gene transcription in lung and uterine ILC2s, whether such changes differed between lung and uterine ILC2s, and compared baseline gene expression between ILC2s from those organs. ILC2s, defined as lineage (CD3e, CD14, CD16/32 and B220) negative, CD25 positive and CD44 high lymphocytes, were sorted by flow cytometry from 8-12 week old BALB/c mice and cultured overnight in RPMI 1640 with 10% charcoal/dextran treated calf serum in the absence or presence of 100 ng/ml estrogen. RNA was extracted from the cells after culture.

ORGANISM(S): Mus musculus

SUBMITTER: Kathleen Bartemes

PROVIDER: E-MTAB-5789 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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