Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

ChIP-seq of Mediator subunits in Mediator mutants and wild type yeast


ABSTRACT: Mediator subunits Med17 (Srb4) and Med15 (Gal11) were subjected to ChIP in wild type yeast, med18 (srb5), med20 (srb2), and med2 med3 med15 (triple mutant) yeast, all in kin28-anchor away yeast after 1 hr rapamycin treatment to evict Kin28 from the nucleus. This results in inactivation of Kin28, which reduces Mediator turnover at promoters and allows stronger Mediator ChIP signal at promoters.

Both Med17 and Med15 were tagged with myc and precipitated using the monoclonal antibody against c-myc. Please note that a specific control for rapamycin treatment was not performed in this experiment, as that has been well documented in the literature for this experiment, using what is called the anchor away method. The untagged sample is a control for non-specific immunoprecipitation.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Randall Morse H 

PROVIDER: E-MTAB-5824 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2010-09-21 | GSE10079 | GEO
2020-04-06 | GSE129898 | GEO
2011-08-31 | E-GEOD-31774 | biostudies-arrayexpress
2013-06-07 | GSE47712 | GEO
2013-06-07 | E-GEOD-47712 | biostudies-arrayexpress
2014-03-24 | GSE55402 | GEO
2018-01-10 | GSE97913 | GEO
2014-03-24 | E-GEOD-55402 | biostudies-arrayexpress
2011-09-01 | GSE31774 | GEO
2018-10-08 | GSE112721 | GEO