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Lotus japonicus response to Nod factor: wild type and nfre mutants

ABSTRACT: For RNA sequencing, 3 days seedlings were moved to agar plates supplemented with 1/4 B&D media and susceptible zone of 14 days-old plants was harvested after specific treatment with water (Mock treated) or M.loti Nod factor 10-8M (NF). The total RNA was isolated from the susceptible zone (15 mm root pieces) using Nucleo spin RNA plant (Macherey-Nagel). Total RNA (> 0.8 g) from two biological replicas per sample was used by GATC Biotech (Germany) to prepare random primed cDNA library and for sequencing with Illumina HiSeq: read length 1 x 50bp. Gifu- L. japonicus wild-type, 4820- nfre-1 allele of Nfre, 38534- nfre-2 allele of Nfre.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Lotus japonicus

SUBMITTER: Simona Radutoiu

PROVIDER: E-MTAB-5855 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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