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Microarray profiling of mESCs differentiation time course toward neuronal lineage

ABSTRACT: mESC adapted to 2i/LIF conditions over four passages (8 days) before differentiation towards the neuronal lineage was induced using experimental conditions described in the literature (PMID: 12524553).

ORGANISM(S): Mus musculus

SUBMITTER: Patrick Stumpf

PROVIDER: E-MTAB-5861 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Stem Cell Differentiation as a Non-Markov Stochastic Process.

Stumpf Patrick S PS Smith Rosanna C G RCG Lenz Michael M Schuppert Andreas A Müller Franz-Josef FJ Babtie Ann A Chan Thalia E TE Stumpf Michael P H MPH Please Colin P CP Howison Sam D SD Arai Fumio F MacArthur Ben D BD

Cell systems 20170901 3

Pluripotent stem cells can self-renew in culture and differentiate along all somatic lineages in vivo. While much is known about the molecular basis of pluripotency, the mechanisms of differentiation remain unclear. Here, we profile individual mouse embryonic stem cells as they progress along the neuronal lineage. We observe that cells pass from the pluripotent state to the neuronal state via an intermediate epiblast-like state. However, analysis of the rate at which cells enter and exit these o ...[more]

PMID: 28957659

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