RNA-seq of coding RNA from DDX6 knockout human haploid (HAP1) cells

ABSTRACT: We performed a genome-scale screen for suppressors of interferon stimulated gene (ISG) expression in human haploid cells (HAP1). DEAD-box helicase 6 (DDX6) was a significant hit. In order to validate DDX6 as a regulator of ISG expression, we created knockouts of DDX6 in HAP1 cells using CRISPR-Cas9 and performed RNA-seq on coding RNA from DDX6 KO and WT cells. This data was used to determine if ISGs were upregulated in DDX6 KO HAP1 cells.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Jennifer Lumb 

PROVIDER: E-MTAB-5867 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress