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RNAseq of roots and leaves of tender wheat (Triticum aestivum cv chinese Spring) during interactions with mycorhizal fungi (Funneliformis mossae) with and without a pathogen attack by Xanthomonas translucens

ABSTRACT: We monitored by RNAseq the transcriptomic response of roots and leaves of Triticum aestivum cv chinese Spring during a long term interaction with Funneliformis mossae (2 months) with or without a pathogen infection by infiltration of Xanthomonas translucens CFBP 2054. The control condition of roots and leaves wheat without mycorhizal fungi is in E-MTAB-5891 (material produced simultaneously and treated at the same time).

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Triticum aestivum

SUBMITTER: Lionel Moulin

PROVIDER: E-MTAB-5898 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Omics approaches revealed how arbuscular mycorrhizal symbiosis enhances yield and resistance to leaf pathogen in wheat.

Fiorilli Valentina V Vannini Candida C Ortolani Francesca F Garcia-Seco Daniel D Chiapello Marco M Novero Mara M Domingo Guido G Terzi Valeria V Morcia Caterina C Bagnaresi Paolo P Moulin Lionel L Bracale Marcella M Bonfante Paola P

Scientific reports 20180625 1

Besides improved mineral nutrition, plants colonised by arbuscular mycorrhizal (AM) fungi often display increased biomass and higher tolerance to biotic and abiotic stresses. Notwithstanding the global importance of wheat as an agricultural crop, its response to AM symbiosis has been poorly investigated. We focused on the role of an AM fungus on mineral nutrition of wheat, and on its potential protective effect against Xanthomonas translucens. To address these issues, phenotypical, molecular and ...[more]

PMID: 29941972

Similar Datasets

RNA-seq of wheat leaves and roots in response to Xanthomonas translucens infection

Project description:This study aimed at decrypting the transcriptomic response of 2 months-old grown tender wheat (cv Chinese Spring) to a the Xanthomonas translucens pathogen infection by infiltration. The response was monitored by RNAseq 24h post leaf clipping. Triticum aestivum cv. Chinese Spring plants were maintained in a growth chamber with cycles of 12 h of light at 21C and 50% relative humidity (RH) and 12 h of dark at 21C and 50% RH. Leaves of 49 days-old plants were infiltrated with a bacterial suspension in water with an optical density at 600 nm (OD600) of 0.5 using a needleless syringe. Plants inoculated with water were used as controls. For transcriptomic and proteomic analyses, leaves and root tissues were harvested 1 day post-inoculation (dpi), when symptoms were not visible yet. Three biological replicates per treatment were performed, and each with pooled leaves from two independent plants per replicate. The files per conditions and replicates are:Sample 1 Root tissue with 3 replicates: CONTROL * control condition for roots (wheat without pathogen infection): 3 replicates: 1.1R,1.2R, 1.3R * control condition for leaves (wheat without pathogen infection): 3 replicates1.1L,1.2L, 1.3L * Wheat Roots infected by Xanthomonas translucens: 3 replicates: 5.1R, 5.2R, 5.3R * Wheat Leaves infected by Xanthomonas translucens: 3 replicates: 5.1L, 5.2L, 5.3L

2017-09-01 | E-MTAB-5891 | biostudies-arrayexpress

Degradome sequencing in Medicago truncatula roots (Glomus intraradices colonized and non-colonized)

Project description:Identification of microRNA targets in M. truncatula roots Degradome sequencing of mycorrhizal and non-mycorrhiza M. truncatula roots

2011-06-16 | E-GEOD-26217 | biostudies-arrayexpress

RNAseq of roots and leaves of tender wheat (Triticum aestivum cv. Chinese Spring) during interaction with plant-growth-promoting rhizobacteria Azospirillum brasilense sp245 and Burkholderia graminis C4D1M

Project description:We monitored the transcriptomic response of roots and leaves of Triticum aestivum (cv Chinese Spring) at 2 months following the root inoculation by Azospirillum brasilense sp245 or Burkholderia graminis C4D1M. Plants were grown in pot containing a solid substrate (sand+soil) and 3 plants per pot, conditions in triplicates, in greenhouse conditions, and inoculated at seedling stage with OD 1 washed bacterial culture.

2022-12-31 | E-MTAB-5896 | biostudies-arrayexpress

Small RNA sequencing in Medicago truncatula roots (Glomus intraradices colonized and non-colonized)

Project description:Identification of microRNA expressed in M. truncatula roots Small RNA sequencing of mycorrhizal and non-mycorrhiza M. truncatula roots

2011-06-16 | E-GEOD-26216 | biostudies-arrayexpress

Gene expression data from arbuscular mycorrhizal and phosphate-treated Medicago truncatula roots.

Project description:Arbuscular mycorrhiza (AM) interactions between plants and Glomeromycota fungi primarily support phosphate aquisition of most terrestrial plant species. To unravel gene expression in Medicago truncatula root colonization by AM fungi, we used genome-wide transcriptome profiling based on whole mycorrhizal roots. We used GeneChips to detail the global programme of gene expression in response to colonization by arbuscular mycorrhizal fungi and in response to a treatment with phosphate and identified genes differentially expressed during this process. Medicago truncatula roots were harvested at 28 days post inoculation with the two different arbuscular mycorrhizal fungi Glomus intraradices (Gi-Myc) and Glomus mosseae (Gm-Myc) under low phosphate conditions (20 µM phosphate) or after a 28 days treatment with 2 mM phosphate in the absence of arbuscular mycorrhizal fungi (2mM-P). As a control, uninfected roots grown under low phosphate conditions (20 µM phosphate) were used (20miM-P). Three biological replicates consisting of pools of five roots were used for RNA extraction and hybridization on Affymetrix GeneChips.

2011-10-28 | E-GEOD-32208 | biostudies-arrayexpress

Transcript abundances in leaves and roots of Populus×canescens inoculated with different ectomycorrhizal fungi in response to drought stress

Project description:Populus×canescens inoculated with or without different ectomycorrhizal fungi, Paxillus involutus (MAJ) and Cenococcum geophilum Fr., were co-cultured in greeenhouse for 16 weeks, then exposed to different water conditions (well-watered, drought and re-watered) for 4 weeks. Poplar leaves and roots were harvest for RNA sequenceing.

2024-03-30 | E-MTAB-12863 | biostudies-arrayexpress

Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

Project description:The experiment aims to analyze the effect of inoculation with Sinorhizobium medicae MD4 of on the transcriptome of the Medicago truncatula roots of N-limited plants. We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. We collected the roots the non-N treated side of the split roots system and we isolated total RNA for RNAseq analysis. Root transcriptomes were analyze before inoculation, at 1 and 2 days post-inoculation.

2021-04-30 | E-MTAB-9932 | biostudies-arrayexpress

Effect of contrasted N regimes on the root transcriptome of Medicago truncatula plants cultivated in split root systems and inoculated for 2, 4 and 7 days with Sinorhizobium medicae

Project description:The experiment aims to analyze the effect of systemic signaling of plant N demand on the transcriptome of Medicago truncatula roots inoculated with Sinorhizobium medicae MD4 at 2, 4, and 7 days post-inoculation (dpi). We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (10 mM NH4NO3 for N-satisfied plants, 0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. To characterize specifically the impact of systemic signaling of N demand, we collected the roots the non-N treated sides of the split roots systems and we isolated total RNA for RNAseq analysis. Root transcriptomes of N-limited and N-satisfied plants were compared at 2, 4 and 7 dpi.

2021-04-30 | E-MTAB-9941 | biostudies-arrayexpress

Transcription profiling of arbuscular mycorrhiza established at 4 weeks post inoculation between Medicago truncatula and Glomus mosseae

Project description:The transcriptome profile of arbuscular mycorrhiza established at 4 weeks post inoculation between Medicago truncatula and Glomus mosseae as well as between Medicago truncatula and Glomus intraradices is compared

2005-06-30 | E-MEXP-218 | biostudies-arrayexpress

Early drought-responsive changes in pepper leaves and roots

Project description:Drought is one of the major factor that limits crop production and reduces yield. To understand the early response of plants under nearly natural conditions, pepper plants were grown in a greenhouse and drought stressed by withholding water for one week. Plants adapted to the decreasing water content of the substrate by adjustment of their osmotic potential in roots by accumulation of raffinose, glucose, galactinol and proline. In contrast in leaves levels of fructose, sucrose and also galactinol increased. Due to the water deficit cadaverine, putrescine, spermidine and spermine accumulated in leaves whereas the concentration of polyamines was reduced in roots. These polyamines are suggested to rather act as stress protectants than for osmotic adjustment. To understand the molecular basis of the response to this early drought stress better, four suppression subtractive hybridisation libraries from leaves and roots were constructed. Microarray technique was used to identify differentially expressed genes. A total of 109 unique ESTs were detected. The diversity of the putative functions of all identified genes confirms the complexity of the plant response to drought stress. Keywords: Transcription profiling Two-condition experiment in roots and leaves, control leaves (CL) vs. drought-stressed leaves (DL) and control roots (CR) vs. drought-stressed roots (DR). Biological replicates: 4 control (1-4), drought-stressed (1-4), independently grown and harvested. One swap replicate per array.

2010-06-20 | E-GEOD-18627 | biostudies-arrayexpress

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