Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

33 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-Seq of murine lung endothelial cells stimulated with IFN-gamma and dexamethasone

ABSTRACT: Glucocorticoids are potent anti-inflammatory drugs prescribed in various diseases such as asthma, rheumatoid arthritis and multiple sclerosis. The effects of glucocorticoids have extensively been studied in immune cells. However, the regulation of inflammation by glucocorticoids in endothelial cells remains poorly understood. In this study, we stimulated murine lung endothelial cell line with dexamethasone (100 nM) and IFN-gamma (20 ng/mL) for 24 hours and extracted RNA for RNA-seq.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Philippe Van den Steen

PROVIDER: E-MTAB-5921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling of human microvascular endothelial cells in systemic sclerosis

Project description:Patients with the diffuse avascular form of Systemic Sclerosis (SSc) and healthy controls (N) were used as sources of microvascular endothelial cells (MVEC). MVEC were obtained from biopsies of involved skin of the hands in 6 SSc patients and from 6 healthy patients undergoing surgery for traumatic events at the hands. The controls were matched by age and sex to the SSc cases. All patients fulfilled the American College of Rheumatology criteria for SSc, as well as the criteria for the diffuse form of the disease.

2005-08-30 | E-MEXP-417 | biostudies-arrayexpress

Angio-modulation in endothelial cells: an unexpected role of desmoglein-2 in regulating actin dynamics and its relevance to angiogenesis deregulation in systemic sclerosis

Project description:Gene expression study of DSG2 silenced human microvascular endothelial cells Affymetrix GeneChip Human Genome U133 Plus 2.0 Array were used to examine differentially expressed genes between normal microvascular endothelial cells and DSG2 silenced microvascular endothelial cells

2012-10-01 | E-GEOD-21547 | biostudies-arrayexpress

Transcription profiling of lymphatic endothelial cells infected with Kaposi's sacoma associated herpes virus

Project description:This SuperSeries is composed of the following subset Series: GSE16353: The profile of cellular and KSHV microRNAs in AIDS_KS biopsies (and normal skin control biopsies) GSE16354: Infection of Lymphatic and Blood Vessel Endothelial Cells (LEC and BEC) with KSHV GSE16355: Lymphatic endothelial cells (LEC) transfected with the KSHV microRNA cluster GSE16356: Lymphatic endothelial cells (LEC) treated with a MAF-targeted siRNA Refer to individual Series

2009-09-30 | E-GEOD-16357 | biostudies-arrayexpress

The effect of corticosterone treatment on the proteome of mouse brain endothelial bEnd.3 cells

Project description:Elevated glucocorticoids feature body’s responses to a variety of psychological and physiological stressors. To understand how glucocorticoids affect brain endothelial cells that form the blood-brain barrier, we applied quantitative proteomics to study changes in the proteome of a mouse brain endothelial cell line, bEnd3, upon acute 24-hour treatment with 5 uM corticosterone in vitro.

2021-03-01 | PXD022166 | Pride

Glucocorticoid Receptor Signaling is Critical for Mouse Corneal Development, Inhibition of Inflammatory Response and Neovascularization of the Cornea

2024-06-27 | GSE213660 | GEO

Glucocorticoid Receptor Signaling is Critical for Mouse Corneal Development, Inhibition of Inflammatory Response and Neovascularization of the Cornea

Project description:Synthetic glucocorticoids are widely prescribed in the treatment of ocular infections and disorders. The actions of glucocorticoids are mediated by the glucocorticoid receptor (GR); however, the molecular and physiological functions of GR signaling in the cornea are poorly understood. In this study, we show that treatment of mice with glucocorticoid eye drops led to a profound regulation of the corneal transcriptome. To determine the global transcriptional profile of GR activity in the cornea, we treated adult wild-type male mice with dexamethasone (a synthetic glucocorticoid) eye drops or vehicle eye drops for 6 hours and then performed RNA sequencing on RNA extracted from whole corneas. All the animals used for this study were adrenalectomized to remove endogenous glucocorticoids. These data demonstrate that a short exposure of glucocorticoids to the cornea results in major changes in the gene expression landscape.

2024-06-27 | GSE213571 | GEO

Role of SIRT1 in human hepatocellular carcinoma

Project description:To investigate the specific roles of SIRT1 in the development of hepatocellular carcinoma, we employed large-scale gene expression analysis to identify the molecular signature that may affect enabling characteristics of cancer cells. Differentially expressed genes were analyzed on the SNU-182 cells transfected with SIRT1 siRNA and recapitulated molecular signatures that related to hallmarks of cancer. SIRT1 expression in hepatocellular carcinoma was analyzed by RT-PCR and western blot. RNA interference-mediated protein knockdown method was used to investigate oncogenic potential of SIRT1 in hepatocelluar carcinoma

2012-03-01 | E-GEOD-32066 | biostudies-arrayexpress

RNAi knock down of MAFin human lymphatic endothelial cells

Project description:Kaposi sarcoma is the most common cancer in AIDS patients and is typified by red skin lesions. The disease is caused by the KSHV virus (HHV8) and is recognisable by its distinctive red skin lesions. The lesions are KSHV-infected spindle cells, most commonly the lymphatic endothelial and blood vessel endothelial cells (LEC and BEC), plus surrounding stroma. The KSHV virus expresses multiple MAF-downregulating microRNA. Here we test the effects of MAF silencing by siRNA in LEC cells using Affymetrix hgu133plus2 chips. Experiment Overall Design: There are n=3 of 1. LEC control cells transfected with a non-targeting siRNA, 2. LEC transfected with a MAF-targeting siRNA

2009-10-10 | E-GEOD-16356 | biostudies-arrayexpress

Transcriptomic and epigenetic analysis of adipose tissue in Cushing Syndrome [mouse]

Project description:Chronic glucocorticoids (GCs) exposure, resulting from endogenous Cushing Syndrome (CS) or prescribed GCs therapy, is associated with a high cardiometabolic burden. Moreover, previous studies have reported persistence of metabolic syndrome features after remission of hypercortisolism and in particular in visceral adipose tissue (VAT). Thus, in this study we want to analyze the transcriptomic alterations in VAT during active and cured CS and correlate these persistent gene expression changes with histone modifications induced by GCs.

2021-09-03 | GSE153934 | GEO

Transcriptomic response of the arcuate nucleus to oral corticosterone treatment

Project description:Glucocorticoids (GCs) are widely prescribed to treat inflammatory and autoimmune conditions. However, patients often develop adverse metabolic effects, including hyperphagia, obesity, and hyperglycemia.The arcuate nucleus has an established role in the central control of energy balance. In this study we examined the transcriptomic response of the arcuate nucleus to oral corticosterone treatment. Gene expression changes were measured after 2 days and 4 weeks of Cort treatment in ad libitum fed C57BL6/J mice.

2019-04-01 | GSE128148 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data