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Timecourse gene expression microarray analysis of bi-allelic targeted Setdb1 mutant mouse ES cells, comparing cells with one inducible conditional (floxed) null allele and a second constitutive null allele, to control mouse ES cell lines.

ABSTRACT: Gene expression microarray analysis of Setdb1 inducible conditional (floxed) mutant (Setdb1^tm1c(EUCOMM)Wtsi/Setdb1^tm1Wcs) compared to control (Setdb1^tm1c(EUCOMM)Wtsi/+) ES cell lines, without 4'OHT treatment, and timecourse samples following a 48 hour 4'OHT treatment. The 4'OHT induces Cre recombination via the Rosa26CreERT2 knock-in system, to convert the tm1c conditional allele to the tm1d null allele. The Setdb1^tm1Wcs allele is a constitutive null allele. Experimental and control Setdb1 ES cells were each treated with 4’OHT for 48 hours, and gene expression was analyzed at days 0 (before treatment), 2 (end of treatment period), 3, 4, and 6 days. Three biological replicates per genotype per timepoint (individual ES clones) were performed. Parental ES cell lines are from the International Knockout Mouse Consortium (IKMC - EUCOMM and KOMP-CSD programmes) and were modified using bi-allelic gene targeting with a targeted trapping vector generated by re-purposing Intermediate Vectors from the IKMC resource.

INSTRUMENT(S): Illumina BeadArray reader

ORGANISM(S): Mus musculus

SUBMITTER: Robert Andrews

PROVIDER: E-MTAB-5931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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