Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Redirection of therapeutic T cells to a bone marrow niche improves their persistence and anti-tumor functions: transcription profiling by array of resting memory OT-I T_CXCR4) or OT-I T_Control cells isolated from the spleen 90 days following DC vaccination.

ABSTRACT: This study reports the improvement in function and antitumor efficacy of CXCR4 overexpression in therapeutic CD8+ T cells. Polyclonal CD8+ T cells from OT-I C57BL/6 (B6) mice were transduced with a modified pMP71 retroviral vector containing murine Cxcr4 and Gfp reporter sequences (T_CXCR4) or with a control vector containing Gfp alone (T_Control), and co-transferred into to B6 mice that were then vaccinated with peptide-pulsed DC. 90 days following DC vaccination, lymphocytes were isolated from the spleen and resting memory OT-I T_CXCR4 and OT-I T_Control cells were FACS sorted to perform gene expression profiling.

ORGANISM(S): Mus musculus

SUBMITTER: Pedro Miguel de Ascensao Santos e Sousa

PROVIDER: E-MTAB-6091 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Peripheral tissues reprogram CD8+ T cells for pathogenicity during graft-versus-host disease.

Santos E Sousa Pedro P Ciré Séverine S Conlan Thomas T Jardine Laura L Tkacz Claire C Ferrer Ivana R IR Lomas Cara C Ward Sophie S West Heather H Dertschnig Simone S Blobner Sven S Means Terry K TK Henderson Stephen S Kaplan Daniel H DH Collin Matthew M Plagnol Vincent V Bennett Clare L CL Chakraverty Ronjon R

JCI insight 20180308 5

Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic stem cell transplantation induced by the influx of donor-derived effector T cells (TE) into peripheral tissues. Current treatment strategies rely on targeting systemic T cells; however, the precise location and nature of instructions that program TE to become pathogenic and trigger injury are unknown. We therefore used weighted gene coexpression network analysis to construct an unbiased spatial map of TE differenti ...[more]

PMID: 29515032

Dataset Information

Redirection of therapeutic T cells to a bone marrow niche improves their persistence and anti-tumor functions: transcription profiling by array of resting memory OT-I T_CXCR4) or OT-I T_Control cells isolated from the spleen 90 days following DC vaccination.

Publications

Peripheral tissues reprogram CD8+ T cells for pathogenicity during graft-versus-host disease.

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