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Single cell sequencing of lung carcinoma

ABSTRACT: Single cell sequencing of 5 dissected lung tumors.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Bram Boeckx

PROVIDER: E-MTAB-6149 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Phenotype molding of stromal cells in the lung tumor microenvironment.

Lambrechts Diether D Wauters Els E Boeckx Bram B Aibar Sara S Nittner David D Burton Oliver O Bassez Ayse A Decaluwé Herbert H Pircher Andreas A Van den Eynde Kathleen K Weynand Birgit B Verbeken Erik E De Leyn Paul P Liston Adrian A Vansteenkiste Johan J Carmeliet Peter P Aerts Stein S Thienpont Bernard B

Nature medicine 20180709 8

Cancer cells are embedded in the tumor microenvironment (TME), a complex ecosystem of stromal cells. Here, we present a 52,698-cell catalog of the TME transcriptome in human lung tumors at single-cell resolution, validated in independent samples where 40,250 additional cells were sequenced. By comparing with matching non-malignant lung samples, we reveal a highly complex TME that profoundly molds stromal cells. We identify 52 stromal cell subtypes, including novel subpopulations in cell types hi ...[more]

PMID: 29988129

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