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RNAseq of mouse presomitic mesoderm segments along the anteroposterior embryo axis.

ABSTRACT: Somitogenesis is the segmentation of the developing embryonic body axis into somites and is guided by oscillating genes, which create waves of expression that travel across the presomitic mesoderm (PSM) from posterior to anterior. Upon arrival of a wave at the PSM's anterior end, a new somite is formed. To identify genes that are expressed in a wave-like pattern we dissected the PSM of four different mouse embryos (pre-turned), separated the left and right sides, and divided each into five segments, from posterior to anterior (sampling sites 1 to 5). Each segment was used to construct libraries for high-throughput RNA-sequencing. For one embryo, we also sequenced two somites.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Ximena Ibarra Soria 

PROVIDER: E-MTAB-6155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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During gastrulation, cell types from all three germ layers are specified and the basic body plan is established <sup>1</sup> . However, molecular analysis of this key developmental stage has been hampered by limited cell numbers and a paucity of markers. Single-cell RNA sequencing circumvents these problems, but has so far been limited to specific organ systems <sup>2</sup> . Here, we report single-cell transcriptomic characterization of >20,000 cells immediately following gastrulation at E8.25  ...[more]

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