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Unknown,Transcriptomics,Genomics,Proteomics

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Quantitative RT-PCR profiling of miRNA expression of non-small cell lung cancer patients against healthy controls

ABSTRACT: Lung cancer (LC) is one of the major cancers, with survival of patients dictated by the time of diagnosis. Cell-free circulating miRNAs have been proposed as candidate biomarkers for non-small cell lung cancer. The goal of this study was to profile the miRNAs in blood plasma of lung cancer patients diagnosed with squamous cell carcinoma (SCC, n=14) or adenocarcinoma (AD, n=6), and healthy individuals (HD) using miRCURY LNA miRNA qPCR Serum/Plasma Panel (Exiqon). Average Cq of detected assays was used for normalization of miRNA expression.

ORGANISM(S): Homo sapiens

SUBMITTER: Ivan Zaporozhchenko

PROVIDER: E-MTAB-6304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profiling of 179 miRNA Expression in Blood Plasma of Lung Cancer Patients and Cancer-Free Individuals.

Zaporozhchenko Ivan A IA Morozkin Evgeny S ES Ponomaryova Anastasia A AA Rykova Elena Y EY Cherdyntseva Nadezhda V NV Zheravin Aleksandr A AA Pashkovskaya Oksana A OA Pokushalov Evgeny A EA Vlassov Valentin V VV Laktionov Pavel P PP

Scientific reports 20180420 1

Lung cancer is one of major cancers, and survival of lung cancer patients is dictated by the timely detection and diagnosis. Cell-free circulating miRNAs were proposed as candidate biomarkers for lung cancer. These RNAs are frequently deregulated in lung cancer and can persist in bodily fluids for extended periods of time, shielded from degradation by membrane vesicles and biopolymer complexes. To date, several groups reported the presence of lung tumour-specific subsets of miRNAs in blood. Here ...[more]

PMID: 29679068

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