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ACE-Scan of the ATP RNA aptamer on 10^12 Gen1 DNA microarray

ABSTRACT: The experiments were carried out to map the ligand binding landscape of various DNA and RNA duplexed aptamer families. Duplexed Aptamer (DA) constructs were engineered from (i) natural and synthetic DNA and RNA aptamers and (i) synthetic oligonucleotide aptamer-complementary elements synthesized on custom DNA microarrays. The aptamers tested consist of the ATP DNA aptamer, the ATP RNA aptamer, the cocaine DNA aptamer, the human alpha-thrombin DNA aptamer, and the natural add riboswitch aptamer from the pathogenic bacteria Vibrio vulnificus. Each duplexed aptamer family consists of 1000's of synthetic constructs, each formed by hybridizing the aptamer with an aptamer-complementary element (ACE) - here, ACEs consisted of various DNA oligonucleotides synthesized as a custom DNA microarray.

INSTRUMENT(S): Agilent Technologies G2565CA microarray scanner

ORGANISM(S): synthetic construct

SUBMITTER: Jeffrey Munzar

PROVIDER: E-MTAB-6376 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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