Unknown,Transcriptomics,Genomics,Proteomics

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Anopheles coluzzii RNAseq

ABSTRACT: RNA deep seq responses of Anopheles gambiae 3 days post arbovirus infection by artificial blood feeding

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Anopheles coluzzii

SUBMITTER: Adrien Pain

PROVIDER: E-MTAB-6443 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Background. The mosquito, Anopheles gambiae, is the primary vector of human malaria, a disease responsible for millions of deaths each year. To improve strategies for controlling transmission of the causative parasite, Plasmodium falciparum, we require a thorough understanding of the developmental mechanisms, physiological processes and evolutionary pressures affecting life-history traits in the mosquito. Identifying genes expressed in particular tissues or involved in specific biological processes is an essential part of this process. Results. In this study, we present transcription profiles for ~82% of annotated Anopheles genes in dissected adult male and female tissues. The sensitivity afforded by examining dissected tissues found gene activity in an additional 20% of the genome that is undetected when using whole-animal samples. The somatic and reproductive tissues we examined each displayed patterns of sexually dimorphic and tissue-specific expression. By comparing expression profiles with Drosophila melanogaster we also assessed which genes are well conserved within the Diptera versus those that are more recently evolved. Conclusions. Our expression atlas and associated publicly available database, the MozAtlas (www.tissue-atlas.org), provides information on the relative strength and specificity of gene expression in several somatic and reproductive tissues, isolated from a single strain grown under uniform conditions. The data will serve as a reference for other mosquito researchers by providing a simple method for identifying where genes are expressed in the adult, however, in addition our resource will also provide insights into the evolutionary diversity associated with gene expression levels among species. MozAtlas is composed of data covering 15 distinct adult tissues with 4 replicates using Affymetrix chips. To provide other researchers with the ability to compare their own experiments with our analyses, we have constructed a database and web-browser for querying tissue expression in Anopheles. This framework will in the future be expanded to include additional tissues and developmental stages, as well as additional species when available.

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Anopheles coluzzii RNAseq

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