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RNA-seq of total lymph node cells from inducible ll7 gene deletion mice

ABSTRACT: Il7fx/KO Rosa26CreERT2 mice (samples 4, 5 and 6) and CreERT –ve littermate controls (samples 1, 2 and 3) were treated with tamoxifen for five days. Three weeks later, RNA was isolated from total lymph nodes of individual mice and gene expression determined by RNAseq.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Benedict Seddon

PROVIDER: E-MTAB-6451 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IL-7-dependent maintenance of ILC3s is required for normal entry of lymphocytes into lymph nodes.

Yang Jie J Cornelissen Ferry F Papazian Natalie N Reijmers Rogier M RM Llorian Miriam M Cupedo Tom T Coles Mark M Seddon Benedict B

The Journal of experimental medicine 20180222 4

IL-7 is essential for the development and homeostasis of T and B lymphocytes and is critical for neonatal lymph node organogenesis because Il7-/- mice lack normal lymph nodes. Whether IL-7 is a continued requirement for normal lymph node structure and function is unknown. To address this, we ablated IL-7 function in normal adult hosts. Either inducible Il7 gene deletion or IL-7R blockade in adults resulted in a rapid loss of lymph node cellularity and a corresponding def ...[more]

PMID: 29472496

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2018-03-31 | E-MTAB-6547 | biostudies-arrayexpress

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