RNA-seq of primary murine (C57BL/6J) LSK cells overexpressing miR-10a or miR-335

ABSTRACT: In this experiment, we want to assess the effect of a lentiviral miR-10a and miR-335 overexpression on the transcriptome of murine LSK (Lin-,Sca-1+,c-Kit+) cells. Primary LSK cells were transduced with lentiviral miRNA overexpression constructs (control: GFP overexpression) and sorted for transduced cells (GFP+) after five days of in vitro culture (Flt-3, TPO, IL-3, SCF containing media).

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Elias Eckert 

PROVIDER: E-MTAB-6496 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress