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ChIP-seq of Mycobacterium smegmatis PafBC to identify in vivo binding sites

ABSTRACT: We aimed to determine the binding sites of the putative transcriptional regulator PafBC under DNA damage stress induced by mitomycin C or under oxidative stress induced by hydrogen peroxide. Therefore, we chose a ChIP-seq approach, crosslinking cells before PafBC-DNA complexes were immunoprecipitated with a PafBC-specific antibody.

INSTRUMENT(S): Ion Torrent PGM

ORGANISM(S): Mycobacterium smegmatis str. MC2 155

SUBMITTER: Andreas Müller

PROVIDER: E-MTAB-6503 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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