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Microarray gene expression analysis of human melanoma A375 cells resistant to 0.5mM phenformin against sensitive naive cells.

ABSTRACT: The aim of this study was to generate an easy-to-reproduce, on-off in vitro model of metabolic switch in melanoma that can be used as a tool for the study of metabolic reprogramming under stress conditions, therapeutic pressure or microenvironmental changes. Experimentally, we forced melanoma cells to adapt to unfunctional OXPHOS by treating them with increasing doses of phenformin up to 0.5mM. We then characterized resistant (R) vs parental (S) cells both at the transcriptional and functional level, in particular focusing on cells aggressiveness and metabolism. By withdrawing phenformin, R cells returned functionally and metabolically similar to S cells, this confirmning the possibility to use this model as an on-off in vitro model of metabolic switch in melanoma.

ORGANISM(S): Homo sapiens

SUBMITTER: federica torricelli

PROVIDER: E-MTAB-6663 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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