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Nuclear run-on sequencing analysis of histone deacetylase inhibition

ABSTRACT: nuclear run-on sequencing analysis of histone deacetylase inhibition

ORGANISM(S): Homo sapiens

DISEASE(S): breast cancer

SUBMITTER: Tae Hoon Kim

PROVIDER: E-MTAB-668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HDAC inhibitors induce transcriptional repression of high copy number genes in breast cancer through elongation blockade.

Kim Y J YJ Greer C B CB Cecchini K R KR Harris L N LN Tuck D P DP Kim T H TH

Oncogene 20130225 23

Treatment with histone deacetylase inhibitors (HDACI) results in potent cytotoxicity of a variety of cancer cell types, and these drugs are used clinically to treat hematological tumors. They are known to repress the transcription of ERBB2 and many other oncogenes, but little is known about this mechanism. Using global run-on sequencing (GRO-seq) to measure nascent transcription, we find that HDACI cause transcriptional repression by blocking RNA polymerase II elongation. Our data show that HDAC ...[more]

PMID: 23435418

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