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Unknown,Transcriptomics,Genomics,Proteomics

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Microarray of isolated adipocytes from subcutaneous adipose tissue of severely obese patients compared to lean controls

ABSTRACT: The aim of the project was to compare global gene expression in adipocytes from obese patients and lean controls. Subcutaneous adipose tissue was collected from severely obese patients undergoing bariatric surgery (average body-mass index (BMI) of 45.5 kg/m2 (n = 12, thereof 4 men) and healthy lean patients undergoing hernia repairs (average BMI of 24.2 kg/m2 (n = 12, thereof 7 men), between 27 and 56 years of age. Adipocytes were isolated by collagenase treatment of adipose tissue, followed by filtering and centrifugation. Floating adipocytes were lysed in Qiazol before RNA purification and microarray analysis.

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Dankel

PROVIDER: E-MTAB-6728 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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IRX5 regulates adipocyte amyloid precursor protein and mitochondrial respiration in obesity.

Bjune Jan-Inge JI Haugen Christine C Gudbrandsen Oddrun O Nordbø Ole P OP Nielsen Hans J HJ Våge Villy V Njølstad Pål R PR Sagen Jørn V JV Dankel Simon N SN Mellgren Gunnar G

International journal of obesity (2005) 20181211 11

<h4>Objective</h4>A causal obesity risk variant in the FTO locus was recently shown to inhibit adipocyte thermogenesis via increased adipose expression of the homeobox transcription factors IRX3 and IRX5. However, causal effects of IRX5 on fat storage remain to be shown in vivo, and discovery of downstream mediators may open new therapeutic avenues.<h4>Methods</h4>17 WT and 13 Irx5 knockout (KO) mice were fed low-fat control (Ctr) or high-fat (HF) diet for 10 weeks. Body weight, energy intake an ...[more]

PMID: 30538277

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