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Bulk RNA-sequencing of self-maintaining versus monocyte-replaced gut macrophages

ABSTRACT: Self-maintaining gMacs are present in the submucosal and myenteric plexus of the intestine where they maintain enteric neurons and submucosal vasculature YFP-positive and YFP-negative CD11b+, CD64+ macrophages were sorted from lamina propria and muscularis externa of Cx3cr1CreERT2.Rosa26-LSL-YFP animals, 35 weeks after tamoxifen administration. Four biological replicates each. YFP-positive and YFP-negative were compared within lamina propria and muscularis externa

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Sebastiaan De Schepper

PROVIDER: E-MTAB-6977 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-cell RNA sequencing of self-maintaining lamina propria macrophages

Project description:Single-cell RNA sequencing of 697 YFP+ CD64+ lamina propria macrophages isolated from Cx3cr1CreERT2.Rosa26-LSL-YFP mice 35 weeks after tamoxifen administration

2018-07-30 | E-MTAB-6976 | biostudies-arrayexpress

Comparison between lamina propria macrophages and muscularis macrophages

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Mass spectrometric detection of proteins associated with lymph node metastasis in T1/2 colorectal cancer from FFPE archival specimens

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2021-01-14 | PXD020808 | Pride

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Project description:Single-cell RNA sequencing using SmartSEQ2 platform of Cx3cr1+ macrophages isolated from the muscularis externa of mice of 10 and of 56 days of age.

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Project description:The following lymphocytes were sorted from the lamin propria of the small intestine of EomesGfg/+ RORgtCreTGg Rosa26Yfp/+ by using the markers Lineage- CD45+ Nkp46+ NK1.1+ : 1.convential NK cells (Eomes GFP+ RORgt YFP-) 2. ILC1 (Eomes GFP- RORgt YFP-) 3. exRORgt ILC3 (Eomes GFP- RORgt YFP+). Conventional NK cells from the bone marrow (cNK BM) were sorted from Eomes Gfp/+ mice with the markers Lineage- CD45+ NK1.1+ Eomes GFP+.

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Resolving Resident Colonic Muscularis Macrophage Diversity and Plasticity During Colitis

Project description:Background: Immune cell populations within the intestinal muscularis propria are poorly resolved during colitis. Maintaining homeostasis in this unique niche is of critical importance, highlighted by the poorer prognosis of inflammatory bowel disease associated with inflammation in the muscularis propria. Methods: This study utilizes single-cell RNA sequencing to survey the immune cell populations within the muscularis propria of normal colon and DSS-induced colitis. Results: In naïve conditions, transcriptional duality is observed in MMφ with two major supopulations: conventional resident Cx3cr1+ MMφs and Lyve1+ MMφs. During colitis, significant changes occur within the muscularis propria, with increases in B-cells, T-cells, monocytes, neutrophils, and dendritic cells. Unlike in the mucosa, single cell transcriptomics indicates that resident MMφs are retained during colitis and exhibit plasticity toward an inflammatory profile. Lyve1+ MMφs, which express anti-inflammatory marker CD163, are absent during colitis. In contrast, resident Cx3cr1+ MMφs remain during colitis. Conclusions: Our findings provide a resource for understanding the immune system in the muscularis propria niche during colitis and resolve the heterogeneity and origins of proinflammatory MMφs during colitis by demonstrating the plasticity of the persistent MMφ population.

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Isobaric labeling-based quantitative proteomics of cells purified from the intestine of Crohn’s disease patients reveals immune cell-epithelial cell crosstalk of potential importance in disease pathogenesis

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2023-01-02 | PXD036591 | Pride

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