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Transcriptional and physiological diurnal responses of Tetradesmus obliquus under light-dark cycles and nitrogen limitation

ABSTRACT: This study is a follow up analysis of Tetradesmus obliquus 16:8 hours diurnal cycle experiments, where a starch-less mutant is compared to the wild-type, but this time, in nitrogen limited condition. The samples were collected approximately every 3 hours for both strains (wild-type and starchless mutant). Two time points per condition have two duplicate set (4 samples) because each condition was done in two batches (allowing to take samples during dark period). Overall, this study contain 41 samples, 20 for the wild-type and 21 for the starchless mutant. RNA was extracted for each samples and sent to Novogene for sequencing.

INSTRUMENT(S): flat panel airlift-loop reactor with a 1.7 L working volume, Maxwell® 16 LEV simplyRNA Tissue kit (Promega), Illumina HiSeq 4000

ORGANISM(S): Tetradesmus obliquus

SUBMITTER: Dirk Martens

PROVIDER: E-MTAB-7016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Draft Genome Sequence of the Oleaginous Green Alga Tetradesmus obliquus UTEX 393.

Carreres B M BM de Jaeger L L Springer J J Barbosa M J MJ Breuer G G van den End E J EJ Kleinegris D M M DM Schäffers I I Wolbert E J H EJ Zhang H H Lamers P P PP Draaisma R B RB Martins Dos Santos V A P VA Wijffels R H RH Eggink G G Schaap P J PJ Martens D E DE

Genome announcements 20170119 3

The microalgae Tetradesmus obliquus is able to maintain a high photosynthetic efficiency under nitrogen limitation and is considered a promising green microalgae for sustainable production of diverse compounds, including biofuels. Here, we report the first draft whole-genome shotgun sequencing of T. obliquus The final assembly comprises 108,715,903 bp with over 1,368 scaffolds. ...[more]

PMID: 28104651

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