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SLIC-CAGE (Super-Low Input Carrier-CAGE) application on mouse primordial germ cells isolated from embryonic day E11.5

ABSTRACT: We developed SLIC-CAGE (Super-Low Input Carrier-CAGE) approach to capture 5'end of RNA polymerase II transcripts from as little as 5-10 ng of total RNA. The dramatic increase in sensitivity compared to existing CAGE methods is achieved by specially designed, selectively degradable carrier RNA. We apply SLIC-CAGE on mouse primordial germ cells embryonic day (E) 11.5 - 2 biological replicates.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Nevena Cvetesic

PROVIDER: E-MTAB-7056 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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