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Nus factors prevent premature transcription termination of bacterial CRISPR arrays

ABSTRACT: We used ChIP-seq to map binding of the CRISPR surveillance complex, Cascade, in a Salmonella enterica serovar Typhimurium strain lacking the gene encoding the endonuclease Cas3. We performed ChIP-seq in strains with wild-type and mutant sequences upstream of the two CRISPR arrays, and in strains with wild-type and mutant nusE genes to determine the impact of Nus factor antitermination on CRISPR array function.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Salmonella enterica subsp. enterica serovar Typhimurium

SUBMITTER: Anne Stringer

PROVIDER: E-MTAB-7242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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