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MRNAseq of Drosophila S2 cells after knockdown of EJC components

ABSTRACT: This study used Drosophila melanogaster S2 cells treated with siRNA against the EJC components mago or eIF4A3, or a non-targeting control siRNA (three samples per condition). mRNA-seq libraries were prepared from the samples and the data was used to examine 'RS-exons' - exons which reconstitute a 5' splice site when they are spliced to the upstream exon. In contrast to mammalian cells, RS-exons are not recursively spliced out from Drosophila transcripts after perturbation of the EJC.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jernej Ule

PROVIDER: E-MTAB-7271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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