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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of round spermatids comparing WT (FVBNJ) to H2A.B3 knock-out mice

ABSTRACT: The histone variant H2A.B3 (H2A.Bbd/H2A.Lap1) has a tissue-specific expression pattern and is found to be most highly expressed during spermatogenesis. In order to investigate the role of H2A.B3 in chromatin packaging and regulation of spermatogenesis we created a line of TALEN-edited FVBN mice in which a deletion was introduced into the single exon of the H2AFB3 gene. The deletion was confirmed by Sanger sequencing and off-target effects investigated using exome sequencing. The deletion is visible in the RNA-Seq data of KO mouse, and the deleterious effect on the H2A.B3 protein has been confirmed using immunohistochemistry.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Sebastian Kurscheid

PROVIDER: E-MTAB-7277 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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