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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Single-cell RNA-seq of mouse developing spinal cord

ABSTRACT: 12 cervicothoracic sections of mouse embryos were dissected at E9.5 (2 replicates), E10.5 (2 replicates), E11.5 (2 replicates), E12.5 (3 replicates), E13.5 (3 replicates). Single-cell RNA-seq libraries were encapsulated, sequenced, aligned, and counted with the 10X Genomics’ Chromium platform and Illumina’s HiSeq 4000 platform.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Julien Delile

PROVIDER: E-MTAB-7320 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single cell transcriptomics reveals spatial and temporal dynamics of gene expression in the developing mouse spinal cord.

Delile Julien J Rayon Teresa T Melchionda Manuela M Edwards Amelia A Briscoe James J Sagner Andreas A

Development (Cambridge, England) 20190327 12

The coordinated spatial and temporal regulation of gene expression in the vertebrate neural tube determines the identity of neural progenitors and the function and physiology of the neurons they generate. Progress has been made deciphering the gene regulatory programmes that are responsible for this process; however, the complexity of the tissue has hampered the systematic analysis of the network and the underlying mechanisms. To address this, we used single cell mRNA sequencing to profile cervi ...[more]

PMID: 30846445

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