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RNA-seq of Atlantic salmon primary hepatocytes treated with alpha-linolenic acid

ABSTRACT: Primary hepatocytes were prepared from Atlantic salmon and grown for 8 days. Data from this experiment was compared to precision cut liver slice (PCLS) culture from Atlantic salmon.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Salmo salar

SUBMITTER: Thomas Harvey

PROVIDER: E-MTAB-7368 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq of precision cut liver slice (PCLS) culture treated with increasing amounts of alpha-linolenic acid and insulin

Project description:This experiment was designed to test the effect of alpha-linolenic acid (ALA) and insulin on liver slices prepared from Atlantic salmon. Liver slices were incubated with increasing concentrations of ALA and insulin from 20µM to 100µM and 10nM to 100nM, respectively. RNA was subsequently sequenced and response was evaluated and compared to the expected response from Atlantic salmon feeding trials. The purpose of this was to evaluate liver slice culture as a cell culture system for studying lipid metabolism in Atlantic salmon.

2019-05-08 | E-MTAB-7366 | biostudies-arrayexpress

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